Interpret HSV ABVIC Test Results

How do I interpret the results of the HSV ABVIC test?


       The flow cytometry plots produced by the HSV ABVIC test are intuitive and easy to understand, and these will be included with your test results. In addition, your test results will be analyzed by our team to offer you one of four simple conclusions:

HSV seronegative:

Click here to see a typical negative result

HSV-1 (+):

Click here to see a typical HSV-1 (+) result

HSV-2 (+):

Click here to see a typical HSV-2 (+) result

HSV-1(+)/2(+):

Click here to see a typical HSV-1(+)/2(+) result

Representative examples of HSV ABVIC Test results that support each of these conclusions is described below.

 


Seronegative Result in HSV ABVIC Test
seronegative-(interpret_results-page)       When a person’s blood is evaluated in the HSV ABVIC Test, the first level of analysis focuses on whether their blood contains IgG antibodies that bind HSV-1+ or HSV-2+ cells to significantly higher levels than uninfected (UI) control cells. When blood from seronegative individuals is mixed with test cells, HSV-1+ and HSV-2+ cells remain at the same low vertical position on the y-axis of flow cytometry plots. This is evident in the total HSV antibody test on the left, as well as in the HSV-1 and HSV-2 specific antibody tests in the middle and on the right, respectively. The t0, t1, and t2 test statistics associated with each test are a measure of the average shift of the test populations of HSV-1+ and/or HSV-2+ cells relative to UI cells (background controls). The rectangles superimposed on each plot depict where the HSV-1+ and/or HSV-2+ cells would be expected in an individual who was HSV-1 or HSV-2 seropositive.


HSV-1 (+) Result in HSV ABVIC Test
HSV-1+-Result-(interpret_results-page)       In persons who are HSV-1 (+), the test for total HSV antibody (data in left column) reveals the individual is HSV-seropositive, but a vertical shift in both HSV-1+ and HSV-2+ cells is observed. To resolve whether the individual is HSV-1 (+), HSV-2 (+), or HSV-1(+)/2(+), it is necessary to turn to the two type-specific tests. After HSV-2 pre-adsorption of serum (data in center column), a high level of HSV-1-specific antibody is observed. The probability this individual is HSV-1-seronegative may be statistically shown to be less than one in a million. Thus, a conclusion is offered that the individual is HSV-1 seropositive. The reciprocal HSV-2-specific antibody test reveals that the individual is HSV-2 seronegative.


HSV-2 (+) Result in HSV ABVIC Test
 HSV-2+-Result-(interpret_results-page)      In persons who are HSV-2 (+), the test for total HSV antibody (data in left column) reveals the individual is HSV-seropositive, but a vertical shift in both HSV-1+ and HSV-2+ cells is observed. To resolve whether the individual is HSV-1 (+), HSV-2 (+), or HSV-1(+)/2(+), it is necessary to turn to the two type-specific tests. After HSV-1 pre-adsorption of the serum (data in right column), a high level of HSV-2-specific antibody is observed. The probability this individual is HSV-2-seronegative may be statistically shown to be less than one in a million. Thus, a conclusion is offered that the individual is HSV-2 seropositive. The reciprocal HSV-1-specific antibody test reveals that the individual is HSV-1 seronegative.


HSV-1(+)/2(+) Result in HSV ABVIC Test
 HSV-1+2+-Result-(interpret_results-page)      In persons who are HSV-1(+)/2(+) (double-positive), the test for total HSV antibody reveals the individual is strongly HSV-seropositive. Although double-positive serum produces unusually strong binding to both HSV-1+ and HSV-2+ cells (data in left column), this data is insufficient to prove that the individual is HSV-1(+)/2(+). Thus, it is necessary to turn to the two type-specific tests. After HSV-1 pre-adsorption of the serum (data in right column), a high level of HSV-2-specific antibody is observed. Thus, a conclusion is offered that the individual is HSV-2 seropositive. Reciprocally, after HSV-2 pre-adsorption of the serum (data in center column), a high level of HSV-1-specific antibodies is observed. Thus, a conclusion is offered that the individual is HSV-1 seropositive. Because the person has type-specific antibodies to both HSV-1 and HSV-2, the data show the individual to be HSV-1(+)/2(+).

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